RESUMO
V. cholerae non-O1 non-O139 serogroups isolated from clinical and environmental sources in Córdoba, Argentina, were analyzed for the presence and expression of virulence genes. Most of the strains studied contained the genes toxR and hlyA, but lacked ctxA, zot, ace, tcpA and stn. The culture supernatants were tested for hemolytic and cytotoxic activity. The enterotoxic potential of the strains was studied in a rabbit ileal loop assay and their genetic profiles were compared by PFGE. The environmental strains varied in their virulence phenotype and showed no-clonal relationships. The clinical strains were highly enterotoxic, hemolytic, proteolytic and showed indistinguishable PFGE profiles, although they differed in their cytotoxic activity. This is the first description, using cell culture and “in vivo” studies, of the virulence properties of non-O1 non-O139 V. cholerae from Argentina.
En este trabajo se analizó la presencia y expresión de genes de virulencia en V. cholerae no-O1 no-O139 de origen clínico y ambiental, aislados en Córdoba, Argentina. La mayoría de las cepas estudiadas contiene los genes toxR y hlyA, pero no ctxA, zot, ace, tcpA y stn. Se analizó la actividad hemolítica y citotóxica de estas cepas en los sobrenadantes de cultivo, así como su potencial enterotóxico en ensayos de asa ileal ligada de conejo. Además, los aislamientos fueron comparados por sus perfiles genéticos en PFGE. Las cepas del medio ambiente mostraron variación en su fenotipo de virulencia y no mostraron relación clonal. Las cepas clínicas fueron muy enterotóxicas, hemolíticas, proteolíticas y mostraron perfiles indistinguibles de PFGE, aunque mostraron diferencias en su actividad citotóxica. En este trabajo se describen por primera vez, utilizando ensayos de cultivo celular e “in vivo”, propiedades de virulencia de V. cholerae no-O1 no-O139 aislados en Argentina.
Assuntos
Animais , Humanos , Coelhos , Vibrio cholerae não O1/patogenicidade , Argentina/epidemiologia , Técnicas de Tipagem Bacteriana , Proteínas de Bactérias/genética , Proteínas de Bactérias/isolamento & purificação , Proteínas de Bactérias/fisiologia , Chlorocebus aethiops , Células COS/microbiologia , Toxina da Cólera/genética , DNA Bacteriano/genética , Farmacorresistência Bacteriana , Diarreia/epidemiologia , Diarreia/microbiologia , Eletroforese em Gel de Campo Pulsado , Enterotoxinas/genética , Enterotoxinas/isolamento & purificação , Enterotoxinas/fisiologia , Deleção de Genes , Genes Bacterianos , Proteínas Hemolisinas/genética , Proteínas Hemolisinas/isolamento & purificação , Proteínas Hemolisinas/fisiologia , Metaloendopeptidases/genética , Metaloendopeptidases/isolamento & purificação , Metaloendopeptidases/fisiologia , Filogenia , Vibrioses/epidemiologia , Vibrioses/microbiologia , Vibrio cholerae não O1/efeitos dos fármacos , Vibrio cholerae não O1/genética , Vibrio cholerae não O1/isolamento & purificação , Virulência/genética , Microbiologia da ÁguaRESUMO
V. cholerae non-O1 non-O139 serogroups isolated from clinical and environmental sources in Córdoba, Argentina, were analyzed for the presence and expression of virulence genes. Most of the strains studied contained the genes toxR and hlyA, but lacked ctxA, zot, ace, tcpA and stn. The culture supernatants were tested for hemolytic and cytotoxic activity. The enterotoxic potential of the strains was studied in a rabbit ileal loop assay and their genetic profiles were compared by PFGE. The environmental strains varied in their virulence phenotype and showed no clonal relationships. The clinical strains were highly enterotoxic, hemolytic, proteolytic and showed indistinguishable PFGE profiles, although they differed in their cytotoxic activity. This is the first description, using cell culture and "in vivo" studies, of the virulence properties of non-O1 non-O139 V. cholerae from Argentina.
Assuntos
Vibrio cholerae não O1/patogenicidade , Animais , Argentina/epidemiologia , Proteínas de Bactérias/genética , Proteínas de Bactérias/isolamento & purificação , Proteínas de Bactérias/fisiologia , Técnicas de Tipagem Bacteriana , Células COS/microbiologia , Chlorocebus aethiops , Toxina da Cólera/genética , DNA Bacteriano/genética , Diarreia/epidemiologia , Diarreia/microbiologia , Farmacorresistência Bacteriana , Eletroforese em Gel de Campo Pulsado , Enterotoxinas/genética , Enterotoxinas/isolamento & purificação , Enterotoxinas/fisiologia , Deleção de Genes , Genes Bacterianos , Proteínas Hemolisinas/genética , Proteínas Hemolisinas/isolamento & purificação , Proteínas Hemolisinas/fisiologia , Humanos , Metaloendopeptidases/genética , Metaloendopeptidases/isolamento & purificação , Metaloendopeptidases/fisiologia , Filogenia , Coelhos , Vibrioses/epidemiologia , Vibrioses/microbiologia , Vibrio cholerae não O1/efeitos dos fármacos , Vibrio cholerae não O1/genética , Vibrio cholerae não O1/isolamento & purificação , Virulência/genética , Microbiologia da ÁguaRESUMO
V. cholerae non-O1 non-O139 serogroups isolated from clinical and environmental sources in Córdoba, Argentina, were analyzed for the presence and expression of virulence genes. Most of the strains studied contained the genes toxR and hlyA, but lacked ctxA, zot, ace, tcpA and stn. The culture supernatants were tested for hemolytic and cytotoxic activity. The enterotoxic potential of the strains was studied in a rabbit ileal loop assay and their genetic profiles were compared by PFGE. The environmental strains varied in their virulence phenotype and showed no clonal relationships. The clinical strains were highly enterotoxic, hemolytic, proteolytic and showed indistinguishable PFGE profiles, although they differed in their cytotoxic activity. This is the first description, using cell culture and [quot ]in vivo[quot ] studies, of the virulence properties of non-O1 non-O139 V. cholerae from Argentina.
